Unbending Notes
转基因主粮安全性问题（1）安全的毒性试验
The cry1Ac-M gene, coding one of Bacillus thuringiensis (Bt) crystal proteins, was introduced into maize H99×Hi IIB genome to produce insect-resistant GM maize BT-38. The food safety assessment of the BT-38 maize was conducted in Sprague-Dawley rats by a 90-days feeding study. We incorporated maize grains from BT-38 and H99×Hi IIB into rodent diets at three concentrations (12.5%, 25%, 50%) and administered to Sprague-Dawley rats (n=10/sex/group) for 90days. A commercialized rodent diet was fed to an additional group as control group. Body weight, feed consumption and toxicological response variables were measured, and gross as well as microscopic pathology were examined. Moreover, detection of residual Cry1Ac-M protein in the serum of rats fed with GM maize was conducted. No death or adverse effects were observed in the current feeding study. No adverse differences in the values of the response variables were observed between rats that consumed diets containing GM maize BT-38 and non-GM maize H99×Hi IIB. No detectable Cry1Ac-M protein was found in the serum of rats after feeding diets containing GM maize for 3months. The results demonstrated that BT-38 maize is as safe as conventional non-GM maize.
Liu P, He X, Chen D, Luo Y, Cao S, Song H, Liu T, Huang K, Xu W. A 90-day subchronic feeding study of genetically modified maize expressing Cry1Ac-M protein in Sprague-Dawley rats.Food Chem Toxicol. 2012 Jun 16;50(9):3215-3221.
（送交者: 漫天的芦花 于 2012-08-05 16:03:15 http://www.rainbowplan.org/bbs/topic.php?topic=193991&select=&forum=1）
Abstract
Maize line 1507, containing event DAS-Ø15Ø7-1 (1507), is a genetically modified (GM) maize plant that expresses the cry1F gene from Bacillus thuringiensis (Bt) sbsp. aizawai and the phosphinothricin-N-acetyltransferase (pat) gene from Streptomyces viridochromogenes throughout the plant including in the grain expression of the Cry1F protein confers in planta resistance to the European corn borer (ECB; Ostrinia nubilalis Hübner: Crambidae) and other lepidopteran pests. Expression of the PAT protein confers tolerance to the herbicidal active ingredient glufosinate-ammonium. The current study evaluated the nutritional performance of rats fed diets containing 1507 maize grain in a subchronic rodent feeding study. The grains in this study, 1507, its near-isogenic control (33P66), and a non-GM commercial hybrid (33J56) contained similar amounts of proximates, amino acids, minerals, anti-nutrients, and secondary metabolites. The subchronic feeding study compared standard toxicology response variables in rats fed diets containing 1507 maize grain with those in rats fed diets containing non-GM maize grains. All diets were prepared according to the specifications of PMI Nutrition International, LLC Certified Rodent LabDiet 5002 (PMI) 5002). Diets were fed ad libitum to Sprague-Dawley rats for approximately 90 days. In-life response variables included indicators of dietary performance and weekly evaluations for clinical signs of toxicity. No toxicologically significant differences were observed in the nutritional performance variables, clinical and neurobehavioral signs, ophthalmology, clinical pathology (hematology, clinical chemistry, coagulation, and urinalysis), organ weights, and gross and microscopic pathology between any pair of treatment groups. These results demonstrate that 1507 maize grain is as safe and as nutritious as non-GM maize grain.
Food Chem Toxicol. 2007 Apr;45(4):551-62. Epub 2006 Oct 4.
Thirteen week feeding study with transgenic maize grain containing event DAS-Ø15Ø7-1 in Sprague-Dawley rats.
MacKenzie SA, Lamb I, Schmidt J, Deege L, Morrisey MJ, Harper M, Layton RJ, Prochaska LM, Sanders C, Locke M, Mattsson JL, Fuentes A, Delaney B. Source DuPont Haskell Laboratory, Newark, DE, USA.
(送交者: 漫天的芦花 于 2012-08-05 16:05:02 http://www.rainbowplan.org/bbs/topic.php?topic=193992&select=&forum=1)
Abstract
With the development of genetically modified crops, there has been a growing interest in available approaches to assess the potential allergenicity of novel gene products. We were not sure whether Cry1C could induce allergy. We examined the protein with three other proteins to determine the potential allergenicity of Cry1C protein from genetically modified rice. Female Brown Norway (BN) rats received 0.1 mg peanut agglutinin (PNA), 1mg potato acid phosphatase (PAP), 1mg ovalbumin (OVA) or 5 mg purified Cry1C protein dissolved in 1 mL water by daily gavage for 42 days to test potential allergenicity. Ten days after the last gavage, rats were orally challenged with antigens, and physiologic and immunologic responses were studied. In contrast to sensitization with PNA, PAP and OVA Cry1C protein did not induce antigen-specific IgG2a in BN rats. Cytokine expression, serum IgE and histamine levels and the number of eosinophils and mast cells in the blood of Cry1C group rats were comparable to the control group rats, which were treated with water alone. As Cry1C did not show any allergenicity, we make the following conclusion that the protein could be safety used in rice or other plants.
Regul Toxicol Pharmacol. 2012 Jul;63(2):181-7. Epub 2012 Apr 6.
Potential allergenicity research of Cry1C protein from genetically modified rice.
Cao S, He X, Xu W, Luo Y, Ran W, Liang L, Dai Y, Huang K.
Source
Laboratory of Food Safety and Molecular Biology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China.
(送交者: 漫天的芦花 于 2012-08-05 16:08:49 http://www.rainbowplan.org/bbs/topic.php?topic=193993&select=&forum=1 )
发贴者 六神花露水 于 2012-08-05 16:20:41 回答：健康小白鼠一般能存活18个月至20个月
有网友评论：这个报告是扯淡，杜邦公司就是做转基因的，他自己的实验室会出具转基因负面报告？有关转基因食品安全报告，只有完全独立的第三方提出才可信，并且需要连续喂养小白鼠三代以上，90天是远远不够的。（送交者: 六神花露水 于 2012-08-05 16:14:33）
本人的评论如下：作为人类主粮，做完小白鼠试验只算动物模型阶段 最后还必须从家畜、家禽肉食链 以及志愿者人群中不少于二代毒理试验中得出安全结论 才能讲安全
当年美国激素牛肉欧盟顶美国40多年就让数据证明美国胡弄，当年瘦肉精就是从家畜、家禽肉食链中证明不安全
而激素牛肉与瘦肉精都是中国农业部那些专家以高科技（人家淘汰后）引入中国推广的。
这就是历史事实。
沈阳
2012年08月05日 16时15分 写于北京家中